- We provide custom designed dual labeled probe set, including one probe and a pair of primers.
- Regular package: one vial of probe 5 OD (HPLC) with 5’FAM (reporter) and 3’ TAMRA (quencher), and two vials of primers 2 OD each (PAGE).
- BHQ: black hole quencher, one kind of nonfluorescent quencher.
| Fluorescence | Excitation maximum (nm) | Emission maximum (nm) |
|---|---|---|
| FAM | 490 | 525 |
| JOE | 520 | 548 |
| Cy3 | 554 | 568 |
| Cy5 | 649 | 666 |
| ROX | 575 | 602 |
| Quencher | Absorption Max, nm | Quenching Range, nm |
|---|---|---|
| TAMRA | 544 | 520-570 |
| BHQ1 | 534 | 480-580 |
| BHQ2 | 579 | 550-650 |
| BHQ3 | 672 | 620-730 |
| 5′-FAM/3′-BHQ1 | 5′-FAM/3′-TAMRA |
| 5′-JOE/3′-BHQ1 | 5′-JOE/3′-TAMRA |
| 5′-ROX/3′-BHQ2 | 5′ -Cy3/3′-BHQ2 |
| 5′ -Cy5/3′-BHQ3 |