Endotoxin <0.1 EU per 1 μg of the protein by the LAL method

Activity Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 106 IU/mg.

Purity >95% as determined by SDS-PAGE analysis. Purified by Ni -NTA chromatography

Form The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

Stability & Storage

Lyophilized protein should be stored at -20°C. This product is stable for one year upon receipt, when handled and stored as instructed. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. Please use within one month after protein reconstitution. Avoid repeated freeze/thaw cycles.