GScript First-Strand Synthesis Kit

品牌

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最多可獲得 37 Goin。

Size: 50 Reactions

Kit Content

Component	Size
Oligo(dT)₂₀	50 μl
5X 1st strand buffer	250 μl
DTT (0.1 M)	100 μl
dNTP mix (10 mM)	50 μl
GScript RTase (200 U/ μl)	50 μl

Storage

1. Store all components at -20°C (non-frost-free).
2. Thaw 5X 1st strand buffer and DTT (0.1 M) at the room temperature just prior to use and refreeze immediately.
3. Thaw the dNTP mix (10 mM) and Oligo(dT)20 on ice prior to use and refreeze immediately.

Cat.No. N/A 分類 PCR & qPCR reagents, 分生試劑 (Molecular Biology)

The GScript First-Strand Synthesis Kit is a recombinant M-MLV RTase expressed in E. coli and purified to homogeneity. It has a lower RNase H activity and a high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 Kb. The GScript RTase has a special activity with its secondary structure, rendering stabilization during the reverse transcription reaction.

Quality Control
The quality of the GScript First-Strand Synthesis Kit is tested on a lot-to-lot basis to ensure consistent product quality.