Quality Control 25 mM dNTP Mix is tested functionally in a DNA polymerase reaction that requires all four deoxyribonucleotides.
Volumes and Concentrations
| 20 μl Final Reaction Volume | |
| Final dNTP Conc | dNTP Volume | Rxns per Kit |
| 0.2 mM | 0.16 μl | 6250 |
| 0.5 mM | 0.4 µl | 2500 |
| 1.0 mM | 0.8 µl | 1250 |
| 1.5 mM | 1.2 µl | 830 |
| 25 μl Final Reaction Volume | |
| Final dNTP Conc | dNTP Volume | Rxns per Kit |
| 0.2 mM | 0.2 μl | 5000 |
| 0.5 mM | 0.5 µl | 2000 |
| 1.0 mM | 1 µl | 1000 |
| 1.5 mM | 1.5 µl | 660 |
| 50 μl Final Reaction Volume | |
| Final dNTP Conc | dNTP Volume | Rxns per Kit |
| 0.2 mM | 0.4 μl | 2500 |
| 0.5 mM | 1 µl | 1000 |
| 1.0 mM | 2 µl | 500 |
| 1.5 mM | 3 µl | 330 |
Volumes and Concentrations, continued
| 25 μl Final Reaction Volume | |
| Final dNTP Conc | dNTP Volume | Rxns per Kit |
| 0.2 mM | 0.8 μl | 1250 |
| 0.5 mM | 2 µl | 500 |
| 1.0 mM | 4 µl | 250 |
| 1.5 mM | 6 µl | 160 |
